This week was long. More hours in the office going over the manuals, protocols, and general research stuff, but even more hours in the lab, as I am now in charge of the fate of the majority of the cells. Every week, I will be seeding a new batch of NT2 cells into as many dishes as possible with 3-4 million cells in each (so I have to count them), leaving enough for another 3-4 million cells to pellet and store for RNA isolation, and enough for a 1:10 dilution into a new flask. So each week will become more and more busy because the number of dishes for which I need to change the media and add uridine and/or retinoic acid to will increase, on top of the already numerous number of dishes and flasks that need to be seeded for different experiments. On the bright side, I have passed the stage where I am in constant fear of messing up months of work worth’s of research!
On Friday after work, I went to Prague – or Praha – in the Czech Republic. They have a different currency there, the koruna I believe, that is worth roughly 1/30th the amount of the Euro, and they speak a completely different language, Czech. I was slightly disappointed that I couldn’t practice my very weak German while there, but at least everyone spoke English; Prague being such a tourist center, English is basically the universal language, so Germans, Spanish, French people, would mostly communicate in English as well.
Prague was quite fun! And very beautiful, as I am learning nearly all European cities are. It had a lot to offer, with its bustling city center, a mystical river splitting the city, and a quite impressive castle (which I learned was actually a Cathedral surrounded by a Palace). Here are some pics: